Below is a link to a research study relating Neuroblastoma to loss of heterozygosity on a particular chromosome. Interesting relationship... check it out by clicking the link below!
Clinical Categories of Neuroblastoma are Associated with Different Patterns of Loss of Heterozygosity of Chromosome Arm 1p
Citation:
Mora,
Jaume, V. Cheung Nai-Kong, et al. "Clinical Categories of
Neuroblastoma are Associated with Different Patterns of Loss of
Heterozygosity on Chromosome Arm 1p." Journal of Molecular Diagnostics.
2.1 (2000): 37-46. Web. 16 Apr. 2012.
<http://www.journals.elsevierhealth.com/periodicals/jmdi/article/S1525-1578(10)60613-7/fulltext>.
Hi all,
ReplyDeleteThe questions concerning your paper are below. My advice is for you to try to divide them up roughly evenly among your group members. You are even welcome to post the answers independently if you'd like; just be sure to reiterate the questions. Please come see me if you need help!
1. State each of Darwin’s four postulates. Fully explain how the generation of treatment-resistant pattern iii Neuroblastoma cells (sensu the first paragraph of the article, page 37) meets each of the four postulates for evolution by natural selection.
2. Use the NCBI website to perform a nucleotide BLAST on the Accession number NM_005378.4 (http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch&SHOW_DEFAULTS=on&LINK_LOC=blasthome0). Paste the accession number into the box, click “others” under Database, and then hit the BLAST button. Scroll down to the Descriptions, and name five different taxa that come up in your results. What does that tell you about this oncogene (MYCN)?
3. Think back to the QTL paper we discussed in class. What 4-5 general steps do quantitative geneticists take to find a candidate gene for a quantitative trait; and more specifically, what steps did these authors take to narrow down the pool of potential genes contributing to NB?
4. The authors assert that genomic instability, whether chromosomal or molecular, leads to LOH. Define loss of heterozygosity and explain how it can cause tumorigenesis. Keep in mind the importance of heterozygosity to a population of organisms and apply that logic to the importance of heterozygosity in a population of cells.
Bonus: Considering your interview with Dr. Mozer and this paper, please briefly describe the influence Dr. LaQuaglia has had on your project.
-Dr. Walker